崔杰,邓素娥,张华,莫志安,连人豪
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广州市石门国家森林公园,广州市石门国家森林公园,广州市石门国家森林公园,广州市石门国家森林公园,广州市石门国家森林公园
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摘要: |
以杉木嫩茎为实验材料,建立组织培养体系。结果表明,杉木嫩茎用HgCl2 试剂消毒11min效果最好,污染率和死亡率极低。最佳诱导培养基为MS+6-BA1.5mg/L+NAA0.5mg/L +3%蔗糖。最适宜杉木组培苗生根的培养基为1/2MS+6-BA0.5mg/L+IBA2.0mg/L+2%蔗糖。移栽30d后,成活率达到88%以上。
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关键词:
杉木;外植体;组织培养
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DOI:
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分类号:
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Tissue Culture and Micropropagation of Cunninghamia lanceolata |
CUI Jie,DENG Sue,ZHANG Hua,MO Zhian and LIAN Renhao
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Shimen National Forest Park of Guangzhou,Guangzhou,Shimen National Forest Park of Guangzhou,Guangzhou,Shimen National Forest Park of Guangzhou,Guangzhou,Shimen National Forest Park of Guangzhou,Guangzhou,Shimen National Forest Park of Guangzhou,Guangzhou
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Abstract: |
The tender stem segments of Cunninghamia lanceolata was used for tissue culture and micropropagation. The results showed that 0.1% mercuric chloride solution (11min) was appropriate, the pollution and mortality rate was low. The best medium for proliferation was MS+6-BA1.5mg/L+NAA0.5mg/L+3%S. the most suitable medium for rooting was1/2MS+6-BA0.5mg/L+IBA2.0mg/L +2%S. After 30days, the survival rate was more than 88%.
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Key words:
Cunninghamia lanceolata;explants;tissue culture
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